Revel Young Ian joined the band for their set-closing Everlong at the Bourbon & Beyond festival in Louisville
Other artists on hand to pay their respects to the late singer and guitarist included members of Pearl Jam, Alice in Chains, Black Sabbath and more.
The Foo Fighters frontman made several appearances at Warren Haynes' Christmas Jam over the weekend.
The gig also featured an appearance by Queen's Roger Taylor, who performed with the Taylor Hawkins-led cover band Chevy Metal.
Grohl plays all seven instruments on the track, which was debuted in a new mini-documentary of the same name.
'Play'—a two-part mini-documentary—features a new 23-minute song in which Grohl plays seven different instruments.